Dr Rachel Webb

NAME	Rachel Webb
Research Status	Non-Clinical Scientist
Academic Section	Obstetrics & Gynaecology
Phone Number	020 87254762
Email	rwebb@sgul.ac.uk
Research Interests	Oocyte and follicle growth and maturation Cell signalling
MAJOR Research Techniques	microinjection, epifluorescence confocal imaging, immunocytochemisty, follicle culture and the dissection and manipulation of different stage oocytes and eggs TLC transfection in both primary and stable cell cultures. mRNA preparation
Minor Research Techniques	RT-PCR, sub-cloning (making competent bacteria, transformation, analytical plasmid isolation and restriction digestion, analysis on agarose gels), high purity plasmid preparation, Western blotting HPLC, sub-cellular fractions. measurement of intracellular Ca2+, cAMP and inositol phosphate levels radio-ligand binding
RECENT PUBLICATIONS — ORIGINAL RESEARCH PAPERS
Devader C, Webb RJ, Thomas GM, Dale L. (2006) Xenopus apyrase (xapy), a secreted nucleotidase that is expressed during early development Gene. Feb 15;367:135-41. Webb R. J., Judah, J.D. and Thomas, G.M.H.(2005) The constitute secretion of albumin from hepatocytes relies on protein tyrosine kinase and phosphatase action.American Journal of Physiology Sep;289(3):C748-56. Rachel J. Webb, Harvinder Bains, Caroline Cruttwell and John Carroll (2002) Gap-junctional communication in mouse cumulus—oocyte complexes: implications for the mechanism of meiotic maturation. Reproduction. 123 41-52. Rachel J. Webb, Felicity Marshall, Karl Swann, & John Carroll. (2002) FSH induces a gap-junction dependent dynamic change in [cAMP] and protein kinase A in mammalian oocytes. Developmental Biology. 246, 441-454
RECENT REVIEWS / BOOK CHAPTERS